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How to Take a Fully Colonized Grain Spawn Bag to Bulk – A How To Guide

How to Take a Fully Colonized Grain Spawn Bag to Bulk – A How To Guide

Derek Bodin |

How to Take a Fully Colonized Grain Spawn Bag to Bulk – Step-by-Step Guide

Expanding your fully colonized grain spawn to bulk substrate is a crucial step in mushroom cultivation. This process allows your mycelium to spread, strengthen, and produce larger, healthier yields. Below is a simple, step-by-step guide to ensure a smooth transition from grain spawn to bulk.


What You’ll Need:

Fully colonized grain spawn bag (100% white with strong mycelium)
Bulk substrate (Coco coir, vermiculite, gypsum, or a pre-mixed substrate)
Monotub or grow bin (With air exchange holes and liner)
Sterile gloves & alcohol wipes (To prevent contamination)
Mixing container or liner (For even distribution)


Step 1: Prepare Your Work Area

Before opening anything, ensure your work area is clean and sanitized. Wipe down all surfaces with isopropyl alcohol (70%) and wear sterile gloves to minimize contamination risks.


Step 2: Break Up Your Colonized Grain Spawn

  • Carefully massage the grain spawn bag to break the colonized grains into smaller pieces. This helps the mycelium spread quickly into the bulk substrate.

  • Cut open the spawn bag with a clean knife or scissors.


Step 3: Mix Grain Spawn with Bulk Substrate

  • In a clean mixing container or directly in your monotub's liner, combine the broken-up grain spawn with your prepared bulk substrate.

  • The ideal ratio is 1:2 or 1:3 (1 part grain spawn to 2-3 parts bulk substrate).

  • Mix gently but thoroughly to evenly distribute the mycelium throughout the substrate.


Step 4: Pack the Mixture into Your Monotub

  • Pour the spawn-substrate mix into your lined monotub or grow bin.

  • Gently level the surface but don’t pack it too tightly—mycelium needs airflow to spread. Finish topping it with the rest of your substrate, NO GRAINS SHOULD BE VISIBLE.

  • Optionally, add a thin top layer of vermiculite to provide extra moisture retention.


Step 5: Gently mist the verm(if used) and the inside of the monotub to ensure proper humidity.

  • Moisten vermiculite layer if used. 

  • Spray inside monotub walls until beads of water start to appear.

  • Open daily and spray the walls if you notice a drop in humidity(if the walls are wet you're good to go, if they're dry, open the tub and spray them and replace the lid.

  • After about a week, check for even white growth across the surface.

I'll post an update to this one as we continue. 

Final Tips for Success:

Always use clean tools and gloves to avoid contamination.
Don’t rush the process—let full colonization happen before fruiting.
Monitor humidity & airflow for the best results.
Be patient! Good things take time, and mushrooms will reward you for it.


By following these steps, you’ll successfully expand your colonized grain spawn into a productive bulk grow. Happy growing! 🍄

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